Bronchogen is strictly for laboratory research and is commonly employed in the following investigational areas:

Transcriptional Regulation in Bronchial Epithelium Research utilizes Bronchogen to study gene expression patterns in bronchial explants and cell cultures. Studies focus on quantifying the synthesis of differentiation markers and functional proteins (such as MUC5AC and surfactant proteins) to evaluate how short peptides influence the secretory phenotype of epithelial cells.

DNA Thermostability and Conformation Biophysical assays employ Bronchogen to measure changes in DNA helix stability. Investigators use differential scanning calorimetry to quantify shifts in DNA melting temperatures, characterizing the peptide’s ability to bind and stabilize chromatin structures against thermal denaturation.

Nuclear Transport and Localization Fluorescence microscopy workflows utilize labeled Bronchogen (e.g., FITC-Bronchogen) to track intracellular trafficking. Researchers measure the kinetics of nuclear entry and nucleolar accumulation in cell lines like HeLa to understand the transport mechanisms of short, charged peptides.

Pathway / Mechanistic Context

The primary mechanistic context for Bronchogen in research settings involves epigenetic control via direct peptide-DNA interactions.

• Sequence-Specific DNA Binding: Bronchogen is investigated for its ability to bind to the major groove of DNA in a sequence-specific manner (specifically 5′-CGCG-3′), potentially blocking or recruiting transcription factors.
• Chromatin Stabilization: The peptide acts by increasing the thermodynamic stability of the DNA helix, which may protect the genome from stress-induced unraveling or damage.
• Gene Promoter Activation: In bronchial models, the peptide is studied for its role in activating promoters for genes involved in mucosal defense and epithelial integrity, distinguishing it from general metabolic enhancers.

Preclinical Research Summary

Published preclinical literature documents investigations of Bronchogen across experimental models for pathway characterization and endpoint measurement:

• HeLa Cell Models: Research indicates that Bronchogen penetrates the plasma and nuclear membranes rapidly, accumulating in the nucleus where it colocalizes with chromatin.
• DNA Melting Assays: In vitro studies demonstrate that the addition of Ala-Asp-Glu-Leu to DNA solutions shifts the melting curve, indicating a statistically significant increase in helix thermostability.
• Bronchial Explant Cultures: Data suggests that Bronchogen treatment modulates the expression of genes responsible for inflammation and tissue structure (e.g., Ki67, MUC5AC) in organotypic cultures of bronchial epithelium, promoting a differentiated cellular state.

Form & Analytical Testing

This material is produced via solid-phase peptide synthesis (SPPS) and supplied as a lyophilized (freeze-dried) powder.

• Lyophilization: Removes water content under vacuum to maintain compound integrity and extend shelf-life.
• Identity Verification: Each lot undergoes Mass Spectrometry (MS) to confirm the presence of peptide components.
• Purity Verification: High-Performance Liquid Chromatography (HPLC) is performed to ensure the product meets the purity standard required for reproducible research data.

Storage & Handling

Stable at room temperature for up to 90 days. For long-term storage, keep at -20C (-4F) or colder. Once mixed with a solvent, the solution must be stored at 4C (39F) and utilized within 30 days. Avoid repeated freeze-thaw cycles.

Referenced Citations

References are provided for informational purposes only and are not clinical claims.

1. Monaselidze, J., et al. (2011). Effect of the Peptide Bronchogen (Ala-Asp-Glu-Leu) on DNA Thermostability. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-011-1146-x
2. Fedoreyeva, L., et al. (2011). Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Moscow). https://doi.org/10.1134/S0006297911110022
3. Khavinson, V., et al. (2014). Peptide Regulation of Gene Expression and Protein Synthesis in Bronchial Epithelium. Lung. https://doi.org/10.1007/s00408-014-9620-7

• Stable at room temperature for up to 90 days. For long-term storage, keep at -20°C (-4°F) or colder.
• Once mixed with a solvent (e.g., bacteriostatic water), the solution must be stored at 4 °C (39°F) and utilized within 30 days. Avoid repeated freeze-thaw cycles, as this degrades the peptide structure.

RESEARCH USE ONLY

This product is intended strictly for laboratory research use only. It is not for human or veterinary use. It is not intended for diagnosis, treatment, cure, or prevention of any disease. All purchases are subject to our Terms of Service and Purity Guarantee.

No COAs available for this product.